Identification of synthetic steroids in river water downstream from pharmaceutical manufacture discharges based on a bioanalytical approach and passive sampling
A bioanalytical approach was used to identify chemical contaminants at river sites located downstream from a pharmaceutical factory, where reproductive alterations in wild fish have been previously observed. By using polar organic compound integrative samplers (POCIS) at upstream and downstream sites, biological activity profiles based on in vitro bioassays revealed the occurrence of xenobiotic and steroid-like activities, including very high glucocorticoid, antimineralocorticoid, progestogenic and pregnane X receptor (PXR)-like activities (mu g standard-EQ/g of sorbent range), and weak estrogenic activity (ng E2-EQ/g of sorbent range). Chemical analyses detected up to 60 out of 118 targeted steroid and pharmaceutical compounds in the extracts. In vitro profiling of occurring individual chemicals revealed the ability of several ones to act as agonist and/or antagonist of different steroids receptors. Mass balance calculation identified dexamethasone, spironolactone, and 6-alpha-methylprednisolone as major contributors to corticosteroid activities and levonorgestrel as the main contributor to progestogenic activities. Finally, RP-HPLC based fractionation of POCIS extracts and testing activity of fractions confirmed identified compounds and further revealed the presence of other unknown active chemicals. This study is one of the first to report environmental contamination by such chemicals; their possible contribution to in situ effects on fish at the same site is suggested.
(Environmental Science and Technology. vol. 48, n° 0013-936X, pp. 3649-3657, 22/04/2026)
INERIS, LPTC, UB, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, SEBIO, INERIS, URCA, ULH, NU, URCA, CNRS
Multiscale patterns in the diversity and organization of benthic intertidal fauna among French Atlantic estuaries
Based on a parallel sampling conducted during autumn 2008, a comparative study of the intertidal benthic macrofauna among 10 estuarine systems located along the Channel and Atlantic coasts of France was performed in order to assess the level of fauna similarity among these sites and to identify possible environmental factors involved in the observed pattern at both large (among sites) and smaller (benthic assemblages) scales. More precisely this study focused on unraveling the observed pattern of intertidal benthic fauna composition and diversity observed at among-site scale by exploring both biotic and abiotic factors acting at the among- and within-site scales. Results showed a limited level of similarity at the among-site level in terms of intertidal benthic fauna composition and diversity. The observed pattern did not fit with existing transitional water classification methods based on fish or benthic assemblages developed in the frame of the EuropeanWater Framework Directive (WFD). More particularly, the coastal plain estuaries displayed higher among-site similarity compared to ria systems. These coastal plain estuaries were characterized by higher influence of river discharge, lower communication with the ocean and high suspended particulate matter levels. On the other hand, the ria-type systems were more dissimilar and different from the coastal plain estuaries. The level of similarity among estuaries was mainly linked to the relative extent of the intertidal "Scrobicularia plana-Cerastoderma edule" and "Tellina tenuis" or "Venus" communities as a possible consequence of salinity regime, suspended matter concentrations and fine particles supply with consequences on the trophic functioning, structure and organization of benthic fauna. Despite biogeographical patterns, the results also suggest that, in the context of the WFD, these estuaries should only be compared on the basis of the most common intertidal habitat occurring throughout all estuarine systems and that the EUNIS biotope classification might be used for this purpose. In addition, an original inverse relation between γ-diversity and area was shown; however, its relevance might be questioned.
(Journal of Sea Research (JSR). vol. 90, n° 1385-1101, pp. 95-110, 22/04/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, LOG, INSU - CNRS, ULCO, CNRS, IRD [Ile-de-France], LOBB, OOB, UPMC, CNRS, UPMC, INSU - CNRS, CNRS, MMS, UM, UN UFR ST, UN, UFR SPB, UN, M2C, UNICAEN, NU, INSU - CNRS, UNIROUEN, NU, CNRS, LERLR / LEROC, COAST, IFREMER, LERBN, COAST, IFREMER, UBO EPE, GEMEL-Normandie, BOREA, UNICAEN, NU, MNHN, IRD, SU, CNRS, UA, STARESO, LIENSs, INSU - CNRS, ULR, CNRS
Single and mixture effects of pesticides and a degradation product on fluvial biofilms
The Morcille River located in the Beaujolais vineyard area (Eastern France) is subjected to strong vine-growing pressure leading to the contamination by a range of herbicides and fungicides of the surrounding freshwater environment. Particularly high concentrations of norflurazon, desmethyl norflurazon and tebuconazole were recorded in spring 2010 at the downstream site of the river. Despite their occurrence in rivers, scarce toxicity data are available for these products, in particular in the case of desmethyl norflurazon (main norflurazon degradation product). Furthermore, the toxicity data are generally available only for single compounds and are issued from single species toxicity tests, leading to a lack of ecological relevance. Consequently, this study was undertaken to evaluate the toxic effects of norflurazon, desmethyl norflurazon and tebuconazole singly and in a ternary mixture on fluvial biofilm. Toxicity tests were performed in microplates for 48 h. Photosynthetic endpointsweremeasured using pulse amplitude-modulated fluorometry; diatom densities and taxonomic composition were determined. After 48 h of exposure, significant effects on optimal quantumyield (Fv/Fm) for desmethyl norflurazon and mixturewere observed.
(Environmental Monitoring and Assessment. vol. 186, n° 0167-6369, pp. 3931-3939, 22/04/2026)
UR EABX, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Video Monitoring and Field Measurements of a Rapidly Evolving Coastal System: the River Mouth and Sand Spit of the Mataquito River in Chile
The understanding of morphological processes controlling the evolution of sand spit reformation after a tsunami impact is a challenging and interesting topic, especially in highly energetic and micro tidal environments. A field campaign performed during December 2012 at the Mataquito River mouth in Chile, allowed us to simultaneously monitor topo-bathymetry evolution, wave climate, tidal range, swash zone dynamics and upper beach face evolution over a portion of its sand spit. A video system was set up for a continuous and long-term monitoring of the evolution of the river mouth and sand spit. Primarily, in this work we focus on the application of a video-derived shoreline detection method to assess shoreline evolution and beach cusps migration at hourly scales. We test the method performance on short-term episodic migration of beach cusps recorded during the campaign. Beach face variations at a daily scale were observed, which can be attributed to the migration of beach cusps in the alongshore direction, and linked to wave forcing and alongshore sediment transport.
(Journal of Coastal Research. vol. SI 70, n° 0749-0208, pp. 639-644, 22/04/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, ECOLA, LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS
Morphodynamic characterisation of the human-impacted Bight of Benin sand barrier coast, West Africa
(Journal of Coastal Research. vol. SI 70, n° 0749-0208, pp. 079-083, 22/04/2026)
CEREGE, IRD, INRA, AMU, CdF (institution), INSU - CNRS, CNRS, ECOLA, LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, OLVAC, LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS
A 1D stabilized finite element model for non-hydrostatic wave breaking and run-up
(. vol. 77, 22/04/2026)
BACCHUS, Inria, UB, CNRS, IMB, UB, Bordeaux INP, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Beach cusp dynamics on a reflective beach
Beach cusps are common feature of steep reflective and intermediate beaches. However very few observations reported double coupled cusp systems. Here we present a data set of observations of a beach exhibiting two sets of beach cusps. Data were collected at Grand Popo Beach (Benin, West Africa) in February 2013. Daily topographic survey along a 380m long stretch of shore allowed observing the dynamic of the two set of beach cusps. At the beginning of the field survey, we clearly observe two sets of cusps : the upper beach cusps system is relatively asymetric with a typical wavelength of about 45 m while the lower beach cusps system is relatively symmetric with a typical wave length slightly shorter (about 35 m). After two days, we measured the total destruction of the lower set of beach cusps while the upper set of beach cusps was only partially des tructed. Data suggest that destruction of the lowerbeach cusp system may be related to persistent accretions conditions and/or calm conditions but probably also to the transition from wave- driven circulation dominated by weak alongshore currents with flash and swash rips, to a second period characterized by dominant longshore current further increasing in speed, and rare swash rips. On the other hand the disappearance of the western upper beach cusp might be related to an accretion pattern and to the merging of two individual features. Our observations, consistent with previous works, suggest that beach cusps certainly arise as a result of some combination of erosion and accretion.
(Journal of Coastal Research. vol. SI 70, n° 0749-0208, pp. 669-674, 22/04/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, ECOLA, LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS, LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS, UAC
Persistent Organic Pollutants in a marine bivalve on the Marennes-Oléron Bay and the Gironde Estuary (French Atlantic coast) -Part 2: Potential biological effects
Contaminant effects on defence responses of ecologically and economically important organisms, such as the Pacific oyster Crassostrea gigas, are likely to influence their ability to resist infectious diseases, particularly at the young stages. The aim of this study was to explore the potential relationships between organic contaminants accumulated in the soft tissues of juvenile oysters, defence responses and physiological condition. Oysters were transplanted during summer and winter periods in different sites in the Marennes-Oléron Bay, the first area of oyster production in France, and in the Gironde Estuary, the biggest estuary in Occidental Europe. Among the battery of biochemical and physiological biomarkers applied in the present work (superoxide dismutase -SOD-, catalase, glutathione peroxidase -GPx-, malondyaldehyde -MDA-, catecholase, laccase and lysozyme in gills, digestive gland, mantle and haemolymph, glycogen, proteins and lipids in the digestive gland and the condition index at the whole organism level), MDA and lysozyme in the digestive gland and SOD, GPx and laccase in plasma contibuted to significantly discriminate sites in which oysters bioaccumulated different levels of heavy polycyclic aromatic hydrocarbons (HPAHs), polychlorobiphenyls (PCBs), polybromodiphenylethers (PBDEs), dichlorodiphenyltrichloroethanes (DDTs) and lindane. These results strengthen the hypothesis that it is possible to differentiate sites depending on their contamination levels and biological effects by carrying out studies with transplanted juvenile oysters. In addition, correlations between antioxidant and immune defence responses and PAH and DDT body burdens in the first area of oyster production in France, the Marennes-Oléron Bay, and where massive oyster mortalities have been reported, suggest that the presence of organic chemical contaminants in the Marennes-Oléron Bay may influence defence responses in juveniles of C. gigas, and, therefore, could influence their ability to resist infectious diseases.
(Science of the Total Environment. vol. 514, n° 0048-9697, pp. 511-522, 22/04/2026)
PUJ, LIENSs, INSU - CNRS, ULR, CNRS, UB, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays
Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10 day-zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals testing. Second, a comparative study of toxicity responses from this exposure protocol was then performed using another OECD species, the Japanese medaka. The potential toxicity of both PAHs was assessed through lethal (e.g. survival, hatching success) and sublethal endpoints (e.g. abnormalities, PMR and EROD) measured at different developmental stages, adapted to the embryonic development time of both species. Regarding effects observed for both species, a suitable artificial sediment composition for PAH toxicity testing was set at 92.5 % dw silica of 0.2-0.5 mm grain size, 5 % dw kaolin clay without organic matter for zebrafish and 2.5 % dw blond peat in more only for Japanese medaka. PAH bioavailability and toxicity were highly dependent on the fraction of organic matter in sediment and of the Kow coefficients of the tested compounds. The biological responses observed were also dependent of the species under consideration. Japanese medaka embryos appeared more robust than zebrafish embryos for understanding the toxicity of PAHs due to the longer exposure duration and it lower sensitivity of sediment physical properties.
(Environmental Science and Pollution Research. vol. 21, n° 0944-1344, pp. 13703 - 13719, 22/04/2026)
LIENSs, INSU - CNRS, ULR, CNRS, IFREMER, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, LPGP, INRA, Biosit : Biologie - Santé - Innovation Technologique
Bioaccumulation of perfluoroalkyl compounds in midge (Chironomus riparius) larvae exposed to sediment
Midge larvae (Chironomus riparius) were exposed to sediments from a deposition sampled at a site along the Rhône River (France) downstream of an industrial site releasing various perfluorinated chemicals. This sediment is characterized by high concentrations of perfluoroundecanoic acid (PFUnA) and perfluorotridecanoic acid (PFTrDA) and a low perfluorooctane sulfonate (PFOS) concentration. Concentrations of 23 perfluoroalkyl compounds, including C4eC14 carboxylate acids, C4eC10 sulfonates, and seven precursors, were analyzed in overlying and pore water, sediment, and larvae. Midge larvae accumulated carboxylate acids (C11eC14), PFOS, and two precursors (perfluorooctane sulfonamide: FOSA and 6:2 fluorotelomer sulfonic acid, 6:2 FTSA). These substances accumulated mainly during the fourth instar larvae exponential growth phase. Accumulation of 6:2 FTSA, PFUnA, and PFOS occured via trophic and tegumentary routes. Other compounds mainly accumulated from food. Kinetics followed a partition model, from which uptake and elimination constants were derived.
(Environmental Pollution. vol. 189, n° 0269-7491, pp. p. 27 - p. 34, 22/04/2026)
UR MALY, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, ECCC