Biogeochemical dynamics related to seasonal changes and biomass-density patterns in rhyzosphere sediments of a Zostera noltii meadow
(29/08/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, LPG-ANGERS, LPG, UA, UN UFR ST, UN, INSU - CNRS, CNRS, CJB, UA, IRD, UB
Evaluation of advanced tertiary treatments for the reduction of refractory micropollutants in treated wastewater
Some refractory micropollutants remain in treated wastewater after secondary treatments. In order to reduce their concentration in treated water, tertiary treatments can be used such as advanced oxidation processes and activated carbon. The efficiency of granular activated carbon in a long term is not well known and advanced oxidation processes have mostly been studied at laboratory scale. Advanced oxidation processes and granular activated carbon pilots were studied in two different wastewater treatment plants in order to determine their efficiency on numerous micropollutants. Both processes were efficient (>90%) on beta blockers and other drugs. The removal of alkylphenols was not complete with UV and hydrogen peroxide. Activated carbon was still efficient after six months functioning 24/24.
(pp. 4, 29/08/2026)
IRSTEA, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, UR MALY, IRSTEA
Occurrence and fate of relevant substances in wastewater treatment plants regarding Water Framework Directive and future legislations
The next challenge of wastewater treatment is to reliably remove micropollutants at the microgram per litre range. During the present work more than 100 substances were analysed through on-site mass balances over 19 municipal wastewater treatment lines. The most relevant substances according to their occurrence in raw wastewater, in treated wastewater and in sludge were identified, and their fate in wastewater treatment processes was assessed. About half of priority substances of WFD were found at concentrations higher than 0.1 μg/L in wastewater. For 26 substances, potential non-compliance with Environmental Quality Standard of Water FrameworK Directive has been identified in treated wastewater, depending on river flow. Main concerns are for Cd, DEHP, diuron, alkylphenols, and chloroform. Emerging substances of particular concern are by-products, organic chemicals (e.g. triclosan, benzothiazole) and pharmaceuticals (e.g. ketoprofen, diclofenac, sulfamethoxazole, carbamazepine). About 80% of the load of micropollutants was removed by conventional activated sludge plants, but about two-thirds of removed substances were mainly transferred to sludge.
(Water Science and Technology. vol. 65, n° 0273-1223, pp. 1179-1189, 29/08/2026)
UR MALY, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Development of q-PCR approaches to assess water quality: Effects of cadmium on gene expression of the diatom Eolimna minima
This study was undertaken to develop molecular tools to assess water quality using diatoms as the biological model. Molecular approaches were designed following the development of a rapid and easy RNA extraction method suited to diatoms and the sequencing of genes involved in mitochondrial and photosystem metabolism. Secondly the impact of cadmium was evaluated at the genetic level by q-PCR on 9 genes of interest after exposure of Eolimna minima diatom populations cultured in suspension under controlled laboratory conditions. Their growth kinetics and Cd bioaccumulation were followed.Population growth rates revealed the high impact of Cd at 100μg/L with total inhibition of growth. These results are linked to the high bioaccumulation values calculated after 14 days of exposure, 57.0±6.3μg. Cd/g. dw and 734.1±70μg. Cd/g. dw for exposures of 10 and 100μg. Cd/L respectively.Genetic responses revealed the impact of Cd on the mitochondrial metabolism and the chloroplast photosystem of E. minima exposed to 10 and 100μg. Cd/L with induction of cox1, 12S, d1 and psaA after 7 days of exposure for the concentration of 100μg. Cd/L and of nad5, d1 and psaA after 14 days of exposure for both conditions.This is the first reported use of q-PCR for the assessment of toxic pollution on benthic river diatoms. The results obtained presage interesting perspectives, but the techniques developed need to be optimized before the design of new water quality diagnosis tools for use on natural biofilms.
(Water Research. vol. 46, n° 0043-1354, pp. 934-942, 29/08/2026)
UR REBX, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Barred-beach morphological control on infragravity motion
A conceptual analysis of the coupling between bars and infragravity waves is performed combining laboratory experiments and numerical modeling. Experiments are carried out in a wave flume with a barred profile. The Boussinesq fully-nonlinear model SERR1D is validated with the laboratory data and a sensitivity analysis is performed next to study the influence on the infragravity wave dynamics of bar amplitude and location, and swash zone slope. A novel technique of incident and reflected motions separation that conserves temporal characteristics is applied. We observe that changing bar characteristics induces substantial variations in trapped energy. Interestingly, a modification of swash zone slope has a large influence on the reflected component, controlling amplitude and phase time-lag, and consequently on the resonant pattern. Variations of trapped infragravity energy induced by changes of swash zone slope reach 25 %. These changes in infragravity pattern consequently affect short-wave dynamics by modifying the breakpoint location and the breaking intensity. Our conceptual investigation suggests the existence of a morphological feedback through the action of evolving morphology on infragravity structures which modulates the action of short-waves on the morphology itself.
(pp. currents.24, 29/08/2026)
LEGOS, IRD, UT3, Comue de Toulouse, INSU - CNRS, CNES, CNRS, PUC, UTFSM, LEGI, UJF, Grenoble INP, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Climatically-controlled siliceous productivity in the eastern Gulf of Guinea during the last 40 000 yr
Opal content and diatom assemblages were analysed in core GeoB4905-4 to reconstruct siliceous productivity changes in the eastern Gulf of Guinea during the last 40 000 yr. Opal and total diatom accumulation rates presented low values over the considered period, except during the Last Glacial Maximum and between 15 000 calendar years Before Present (15 cal. ka BP) and 5.5 cal. ka BP, the so-called African Humid Period, when accumulation rates of brackish and freshwater diatoms at the core site were highest. Conversely, accumulation rates of windblown diatoms exhibited an opposite pattern with higher values before and after the African Humid Period and greatest values during Heinrich Events, the Younger Dryas and since 5.5 cal. ka BP. Our results demonstrate that siliceous productivity in the eastern Gulf of Guinea was directly driven by the nutrient load from local rivers, whose discharges were forced by precipitation changes over western Equatorial Africa and/or modification of the fluvio-deltaic systems forced by sea level changes. Precipitation in this region is controlled by the West African monsoon which is, in turn, partly dependent on the presence and intensity of the Atlantic Cold Tongue (ACT). Our results therefore suggest that the ACT was weakened, warmer trade winds were less vigorous, and cloud convec-tion and precipitation were greater during the AHP though centennial-to-millennial timescale dry events were observed at ∼10 cal. ka BP, ∼8.5 cal. ka BP and ∼6 cal. ka BP. Conversely , the ACT was more intense, trade winds were more vigorous and African climate was more arid during H1, the Younger Dryas and after 5.5 cal. ka BP into the present.
(Climate of the Past. vol. 8, n° 1814-9324, pp. 415-431, 29/08/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, IACT, CSIC, UGR, CAU
Vegetation dynamics in the Northeastern Mediterranean region during the past 23 000 yr: insights from a new pollen record from the Sea of Marmara
Abstract. High-resolution pollen analysis of core MD01-2430 from the Sea of Marmara (40°47.81' N, 27°43.51' E) allows us to reconstruct the vegetation response to climatic changes during the past 23 cal ka in the Northeastern Mediterranean. Variation in mesic/temperate forest cover indicates major climatic shifts connected to Heinrich Stadial 1, Bölling-Allerød, Younger Dryas and to the onset of the Holocene. Pollen–anthropogenic indicator approach was used to recognize human-induced landscape changes in the Sea of Marmara. The pollen-inferred onset of the Holocene occurs at ca. 11.5 cal ka, indicating that the Northeastern Mediterranean region represents a transitional zone where higher moisture availability supported an earlier forest expansion than the borderlands of the Aegean Sea and Black Sea. Two major forest retreats occurred during the Holocene at ca. 5.5 and 2.1 cal ka. The Holocene forest setbacks are in phase with previously published alkenone-inferred sea-surface temperature decreases in the Sea of Marmara reconstructed from the same core. Our new pollen record testifies the sensitivity of Mediterranean forests to changes in moisture availability, which is driven by changes in high-latitude atmospheric processes (North Atlantic Oscillations and/or Siberian High).
(Climate of the Past. vol. 8, n° 1814-9324, pp. 1941-1956, 29/08/2026)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, UMR ISEM, Cirad, IRD, EPHE, PSL, UM, CNRS
Seasonal succession of estuarine fish, shrimps, macrozoobenthos and plankton: physico-chemical and trophic influence. The ironde estuary as a case study
Characterization of the structure and seasonal variability of biotic communities is essential for a better understanding of estuarine ecosystem functioning and in order to manage these highly fluctuant and naturally stressed systems. Numerous studies have investigated the role of environmental factors in controlling temporal variations in biotic communities. However, most have concluded that the explanatory power of physico-chemical variables was significant but not sufficient to explain ecological dynamics. The aim of the present study was to put forward the importance of trophic interactions as an additional structuring factor of species seasonal variability by examining simultaneous dynamics of all estuarine biotic communities, with the example of the oligo-mesohaline area of the Gironde estuary (SW France) as a case study. Data on the main biological groups (fish, shrimps, macrozoobenthos and plankton) sampled during a five-year period (2004-2008) at monthly intervals using a well standardized protocol, as well as data on environmental variables, were compiled here for the first time. According to species composition, results indicated that the Gironde estuary is used as a nursery, feeding, resident and migratory habitat. Results showed that, for almost all species, strong seasonal fluctuations occurred with a succession of species, indicating an optimization of the use of the available resources over a typical year by estuarine biological communities. Multivariate analyses discriminated four seasonal groups of species with two distinctive ecological seasons. A clear shift in July indicated a biomass transfer from a “planktonic phase” to a “bentho-demersal phase”, corresponding to spring and summer-autumn periods, respectively. In regard to the temporal fluctuations of dominant species of all biological groups, this study highlighted the possible influence of trophic relationships, predation in particular, on seasonal variations in species abundance, in addition to the physico-chemical influence. This study enabled us to synthesize crucial seasonal data and to discuss their integration into seasonal models of estuarine functioning and/or specific prey-predator models. In a global change context, prey abundance variations could generate changes in the temporal dynamics of their predators (and conversely), and potentially in the functioning of the whole estuarine system.
(Estuarine, Coastal and Shelf Science. vol. 112, n° 0272-7714, pp. 243-254, 29/08/2026)
UR EPBX, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Remediation of a watershed contaminated by heavy metals: A 2-year field biomonitoring of periphytic biofilms
This study focuses on an industrial contamination site subjected to remediation processes since 2007 in the Riou-Mort watershed (southw st France). The purpose was to assess the first impacts of remediation on periphytic biofilms, and was performed during two years of biomonitoring. Periphytic biofilms were collected on glass slides immersed 24 days at different sites along the contamination gradient for 12 colonisation cycles. Metal contaminations (Cd and Zn) were analysed in biofilms and the evolution of diatom communities was assessed, integrating teratology quantifications. Despite remediation work initiated at the industrial site, this study demonstrated the persistence of metal contamination in water, as well as in biofilms. In addition, our data, showed that the remediation process was initially marked by an increase in metal contamination in the river, with increasing diatom community shifts. Metal-contaminated biofilms presented decreasing species diversities and were dominated by metal-resistant species such as Eolimna minima, whom abundances increased in 2010 reaching 57.2 ± 10%. No significant decrease in metal accumulation was observed and total Cd content in biofilms collected downstream the industrial site ranged from 772.7 ± 88 in July 2009 to 636.9 ± 20 μg/gDW in July 2 010. Results obtained on artificial substrates were compared with those of natural substrates and showed similar diatom communities and abundances of deformed diatoms but lower diversities. This ensured that glass slide subtrates gave a good representation of periphytic biofilm health . Finally, results were compared to studies performed before the remediation process and this did not reveal a decrease of metal accumulation in biofilms nor shifts in taxonomic composition of the communities, rather the remaining dominance of metal-resistant species such as E. minima was confirmed.
(Science of the Total Environment. vol. 425, n° 0048-9697, pp. 242-253, 29/08/2026)
UR REBX, IRSTEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Evidence for a Complex Relationship between Antibiotics and Antibiotic-Resistant Escherichia Coli: From Medical Center Patients to a Receiving Environment
The aim of this study was to investigate the relationship between antibiotics and antibiotic-resistant fecal bacteria (E. coli) in water along a medical center−wastewater treatment plant−river continuum (4 km). A multiresidue chemical analysis methodology, using solid phase extraction coupled with liquid chromatography tandem mass spectrometry, was performed to detect whether low levels of contamination by 34 antibiotics were related to antibiotic resistance of E. coli and antibiotic use. The contamination of water by antibiotics and antibiotic-resistant E. coli decreased along the continuum. Although amoxicillin was predominantly prescribed, only ofloxacin (1 ng*L−1) and sulfamethoxazole (4 ng*L−1) persisted in the river. At the retirement home, in the medical center, even though no tetracycline and sulfamethoxazole were consumed, the highest occurrences of antibiotic resistance were in classes of quinolones (42.0%), sulfonamides (24.0%), tetracyclines (38.0%), and penicillins (38.0%), mainly due to the presenceof multiple antibiotic-resistance genes on class 1 integrons. Along the continuum, the occurrence of E. coli resistant to antibiotics and those carrying class 1 integrons decreased in water samples (p-value <0.001). Interestingly, in the river, only persistent antibiotic compounds (ofloxacin, sulfamethoxazole) were found, but they did not correspond to the major resistances (tetracycline, amoxicillin) of E. coli.
(Environmental Science and Technology. vol. 46, n° 0013-936X, pp. 1859−1868, 29/08/2026)
M2C, UNICAEN, NU, INSU - CNRS, UNIROUEN, NU, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, SCALE, ULH, NU, UNIROUEN, NU, CNRS