Improving the chronology of marine cores: IRSL dating and Bayesian modelling of a core from the Bay of Biscay (NE Atlantic)
(13/09/2021)
IRAMAT-CRP2A, IRAMAT, UTBM, UO, UBM, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, EPHE, PSL, LMJL, UN UFR ST, UN, CNRS
Revision of the French Terebellidae sensu stricto (Annelida, Terebelliformia), with descriptions of nine new species
This work is the last of four papers of the Spaghetti Project, aiming to revise the species of terebellids, a.k.a. “spaghetti” worms, present in the European French waters. In this last paper the Terebellidae, sensu stricto, from French waters are revised based, on material available in the French marine stations, type materials stored in the MNHN collection and newly collected specimens. Nine new species are described using both morphological and molecular tools: Eupolymnia gili n. sp., E. lacazei n. sp., E. meissnerae n. sp., Lanice kellyslateri n. sp., Paramphitrite dragovabeci n. sp., Pista labruneae n. sp., P. miosseci n. sp., P. sauriaui n. sp., and Terebella banksyi n. sp. European species of Eupolymnia are distinguished mainly by the shape of the lateral lobes and the size of the branchial stems. The two species belonging to Lanice genus are distinguished by the fusion of the first ventral shields, the shape of both noto- and neuropodia, and the pigmentation of the upper lip. The two species of Paramphitrite are distinguished by the presence or absence of a medial dorsal gap between the pairs of branchiae, by the shape of the lateral lobes and the presence or absence of a nephridial papilla on segment 4. The different species of Pista are distinguished by the number of pairs of branchiae, the shape of the lateral lobes and uncini. Finally, the two species of Terebella are distinguished by the number of segments with nephridial and genital papillae and the segments on which the branchiae occur. An identification key for European species of Terebellidae sensu stricto is also provided.
(Zootaxa. vol. 5038, n° 1175-5326, pp. 1-63, 13/09/2021)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, UMS POREA, INSU - CNRS, CNRS, INRAE
Does temperature affect soil organic matter decomposition and dissolved organic matter optical properties ?
(pp. 1-2, 12/09/2021)
UMR ISPA, Bordeaux Sciences Agro, INRAE, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
A diffusion cell adapted to nuclear imaging instruments for the measurement of molecular release and pharmacokinetics across membranes
Diffusion cells are routinely used in pharmacology to measure the permeation of pharmaceutical compounds and contaminants across membranes (biological or synthetic). They can also be used to study drug release from excipients. The device is made of a donor (DC) and an acceptor (AC) compartment, separated by a membrane. Usually, permeation of molecules across membranes is measured by sampling from the AC at different time points. However, this process disturbs the equilibrium of the cell. Furthermore, analytical techniques used in association with diffusion cells sometimes lack either accuracy, sensitivity, or both. This work reports on the development of nuclear imaging – compatible diffusion cells. The cell is made of a polymer transparent to high-energy photons typically detected in positron emission tomography (PET). It was tested in a finite-dose set-up experiment with a pre-clinical PET system. Porous cellulose membranes (3.5, 25 and 300 kDa), a common excipient in pharmacology, as well as for dialysis membranes, were used as test membranes. The radioisotope 89Zr chelated with deferoxamine B (DFO; 0.65 kDa), was used as an imaging probe (7–10 MBq; 0.2–0.3 nMol 89Zr-DFO). In medicine, DFO is also commonly used for iron removal treatments and pharmacological formulations often require the association of this molecule with cellulose. Permeation profiles were obtained by measuring the radioactivity in the DC and AC for up to 2 weeks. The kinetic profiles were used to extract lag time, influx, and diffusion coefficients of DFO across porous cellulose membranes. A sensitivity threshold of 0.005 MBq, or 3.4 fmol of 89Zr-DFO, was revealed. The lag time to permeation (τ) measured in the AC compartment, was found to be 1.33, 0.5, and 0.19 h with 3.5, 25, and 300 kDa membranes, respectively. Diffusion coefficients of 3.65 × 10−6, 8.33 × 10−6, and 4.74 × 10−5 cm2 h−1 where revealed, with maximal pseudo steady-state influx values (Jpss) of 6.55 × 10−6, 1.76 × 10−5, and 1.29 × 10−5 nmol cm−2 h−1. This study confirms the potential of the technology for monitoring molecular diffusion and release processes at low concentrations, high sensitivities, in real time and in a visual manner. © 2021 Elsevier B.V.
(Journal of Controlled Release. vol. 337, pp. 661-675, 10/09/2021)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Uncertainties in Shoreline Projections to 2100 at Truc Vert beach (France): Role of Sea-Level Rise and Equilibrium Model Assumptions
Ensemble-based simulations of future shoreline evolution to 2100, including sea-level rise driven erosion, are performed and analysed Future shoreline projections uncertainties are initially controlled by modelling assumptions and after 2060 by sea-level rise uncertainties The choice of wave-driven equilibrium modelling approach and incident wave chronology are critical to future shoreline projections 1 Abstract Most sandy coasts worldwide are under chronic erosion, which increasingly put at risk coastal communities. Sandy shorelines are highly dynamic and respond to a myriad of processes interacting at different spatial and temporal scales, making shoreline predictions challenging, especially on long time scales (i.e. decades and centuries). Shoreline modelling inherits uncertainties from the primary driver boundary conditions (e.g. sea-level rise and wave forcing) as well as uncertainties related to model assumptions and/or misspecifications of the physics. This study presents an analysis of the uncertainties associated with future shoreline evolution at the high-energy, cross-shore transport dominated, sandy beach of Truc Vert (France) over the 21 st century. We explicitly resolve wave-driven shoreline change using two different equilibrium modelling approaches to provide new insight into the contributions of sea-level rise, and free model parameters uncertainties on future shoreline change in the frame of climate change. Based on a Global Sensitivity Analysis, shoreline response during the first half of the century is found to be mainly sensitive to the equilibrium model parameters, with the influence of sea-level rise emerging in the second half of the century (~2050 or later), in both Representative Concentration Pathways 4.5 and 8.5 scenarios. The results reveal that the seasonal and interannual variability of the predicted shoreline position is sensitive to the choice of the wave-driven equilibrium based model. Finally, we discuss the importance of the chronology of wave events in future shoreline change, calling for more continuous wave projection time series to further address uncertainties in future wave conditions.
(08/09/2021)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, BRGM, NOC
Benthic oxygen dynamics and implication for the maintenance of chronic hypoxia and ecosystem degradation in the Berre lagoon (France)
Chronic hypoxia and anoxia have strong impacts on coastal aquatic ecosystems worldwide. Such situations are essentially driven by high benthic oxygen (O2) demand resulting from organic matter mineralization in surface sediment and amplified by a low mixing of the water column. However, the benthic O2 demand may greatly vary according to the O2 availability, sediment biogeochemical properties, and bioturbation by macrobenthic fauna. Here we examined how the sediment O2 demand vary in response to seasonal and long-term (pluri-decadal) hypoxia in the Berre lagoon, a coastal ecosystem impacted by chronic hypoxia events since ca 60 years. Oxygen penetration depth, diffusive and total O2 fluxes were measured in situ using a microelectrode autonomous profiler and benthic chamber deployments at three sites impacted by quite-permanent (PA), seasonal (PI) and occasional (PO) hypoxia in August 2016. Those measurements were seasonally repeated at site PI between August 2015 and August 2016. Additional physical and chemical characteristics were also measured in surface sediment. Sediment profile images analysis combined to measures of benthic macrofaunal communities characteristics were determined in order to estimate the quality of the benthic ecosystem. The highest benthic O2 demand was observed after seasonal anoxia in relation to the important accumulation of reduced chemical species in surface sediment. Interestingly, both pluri-decadal hypoxia and normoxia produced relatively high benthic O2 demand related to a higher accumulation of organic matter and to the presence of reduced chemical species at site dominated by hypoxia, and to the presence of fresher organic matter and active bioturbating macrofaunal communities in normoxic site. The low benthic O2 demand at site seasonally impacted by hypoxia was explained by the degraded state of the macrofaunal community and by the lower accumulation of reduced chemical species. The occurrence of hypoxia and anoxia situations in the Berre lagoon was predicted from the competition between kinetics of benthic O2 demand and water column reoxygenation events induced by strong wind. The good correspondence between the measured and predicted hypoxia/anoxia occurrence clearly indicates that the chronic deoxygenation events in the Berre lagoon, and the associated degraded state of the benthic ecosystem are driven jointly by the benthic O2 demand and the intensity and duration of the water column stratification.
(Estuarine, Coastal and Shelf Science. vol. 258, n° 0272-7714, pp. 107437, 05/09/2021)
CHROME, UNIMES, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, MIO, IRD, AMU, INSU - CNRS, UTLN, CNRS, LUSAC, UNICAEN, NU, INTECHMER, Cnam, IMBE, AU, AMU, CNRS, CEREGE, IRD, AMU, CdF (institution), INSU - CNRS, CNRS, INRAE, GIPREB, UFRJ, IRSN/PSE-ENV/SRTE/LRTA, IRSN/PSE-ENV/SRTE, IRSN
Development of duplex TaqMan-based real-time PCR assay for the simultaneous detection of Perkinsus olseni and P. chesapeaki in host Manila clam tissue samples
The aetiological agent Perkinsus olseni is globally recognised as a major threat for shellfish production considering its wide geographical distribution across Asia, Europe, Australia and South America. Another species, Perkinsus chesapeaki, which has never been known to be associated with significant mortality events, was recently detected along French coasts infecting clam populations sporadically in association with P. olseni. Identifying potential cryptic infections affecting Ruditapes philippinarum is essential to develop appropriate host resource management strategies. Here, we developed a molecular method based on duplex real-time quantitative PCR for the simultaneous detection of these two parasites, P. olseni and P. chesapeaki, in the different clam tissues: gills, digestive gland, foot, mantle, adductor muscle and the rest of the soft body. We firstly checked the presence of possible PCR inhibitors in host tissue samples. The qPCR reactions were inhibited depending on the nature of the host organ. The mantle and the rest of the soft body have a high inhibitory effect from threshold of host gDNA concentration of 2 ng.µL − 1 , the adductor muscle and the foot have an intermediate inhibition of 5 ng.µL − 1 , and the gills and digestive gland do not show any inhibition of the qPCR reaction even at the highest host gDNA concentration of 20 ng.µL − 1. Then, using the gills as a template, the suitability of the molecular technique was checked in comparison with the Ray's Fluid Thioglycolate Medium methodology recommended by the World Organisation for Animal Health. The duplex qPCR method brought new insights and unveiled cryptic infections as the co-occurrence of P. olseni and P. chesapeaki from in situ tissue samples in contrast to the RFTM diagnosis. The development of this duplex qPCR method is a fundamental work to monitor in situ co-infections that will lead to optimised resource management and conservation strategies to deal with emerging diseases.
(Journal of Invertebrate Pathology. vol. 184, n° 0022-2011, pp. 107603, 02/09/2021)
LEMAR, IRD, IFREMER, UBO EPE, CNRS, UPVD, CRIOBE, UPVD, EPHE, PSL, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, SGMM, IFREMER
Ultra-trace interference-free analysis of palladium in natural waters by ICP-MS after on-line matrix separation and pre-concentration
The determination of palladium (Pd) in environmental samples by ICP-MS is challenging as all its isotopes are extensively interfered due to isobaric (e.g. 110Cd on 110Pd, 106Cd on 106Pd), polyatomic (e.g. 92Mo16O on 108Pd, 89Y16O on 105Pd) and doubly-charged (e.g. 208Pb2+ on 104Pd) species formed in the plasma from elements usually present at concentrations several orders of magnitude higher. As a result, the determination of Pd in natural waters is extremely scarce despite is has been proven that this metal is subject to a significant anthropogenic impact mainly linked to its use in catalytic converters in motor vehicles. In order to overcome this situation, we have developed an ultra-trace interference-free methodology for the determination of Pd in natural waters by ICP-MS after on-line matrix separation and preconcentration. The method is based on the strong affinity of Pd towards a commercially-available carboxymethylated polyethylenimine resin, which also has the ability to retain most of the transition metals. However, Pd is not eluted from the resin at typical elution conditions (e.g. 2 M HNO3, which removes all the interference-forming metals), but this can be attained by passing a diluted thiourea solution (10−3 M). Therefore, the interference-free on-line determination of Pd in natural waters was successfully achieved using a two-step elution procedure. Procedural blank values were 0.012 ± 0.003 ng kg−1 (n = 6), which results in a detection limit of 0.010 ng kg−1, allowing the determination of dissolved Pd in natural samples at low, ambient concentrations. The optimized methodology was applied to determine the concentrations of Pd in the Gironde estuary, which represents the first dissolved Pd profile along an estuarine salinity gradient and one of the first dataset of Pd concentrations in natural waters at ambient levels in almost 4 decades. The simplicity of the preconcentration setup and the possibility for its automation offers new analytical opportunities, which will be useful for future studies aiming to improve our understanding of the behavior of Pd in natural waters.
(Talanta. vol. 232, n° 0039-9140, pp. 122289, 01/09/2021)
EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
Presentation and evaluation of the IPSL‐CM6A‐LR ensemble of extended historical simulations
The Institut Pierre-Simon Laplace Climate Modeling Center has produced an ensemble of extended historical simulations using the IPSL-CM6A-LR climate model. This ensemble (referred to as IPSL-EHS) is composed of 32 members over the 1850–2059 period that share the same external forcings but differ in their initial conditions. In this study, we assess the simulated decadal to multidecadal climate variability in the IPSL-EHS. In particular, we examine the global temperature evolution and recent warming trends, and their consistency with ocean heat content and sea ice cover. The model exhibits a large low-frequency internal climate variability. In particular, a quasi-bicentennial mode of internal climate variability is present in the model and is associated with the Atlantic Meridional Overturning Circulation. Such variability modulates the global mean surface air temperature changes over the historical period by about $\sim$0.1K. This modulation is found to be linked to the phase present in the initial condition state of each member. This variability appears to decrease during the 1850–2018 period in response to external forcings. The analysis of the ocean heat content reveals furthermore an overestimation of the ocean stratification, which likely leads to an overestimation of the recent warming rate on average
(Journal of Advances in Modeling Earth Systems. vol. 13, n° 1942-2466, pp. e2021MS002565, 01/09/2021)
IPSL (FR_636), ENS-PSL, PSL, UVSQ, CEA, INSU - CNRS, X, IP Paris, CNES, SU, CNRS, UPCité, LOCEAN-NEMO R&D, LOCEAN, MNHN, IRD, INSU - CNRS, SU, CNRS, IPSL (FR_636), ENS-PSL, UVSQ, CEA, INSU - CNRS, X, CNES, SU, CNRS, UPCité, LOCEAN-VARCLIM, LOCEAN, MNHN, IRD, INSU - CNRS, SU, CNRS, IPSL (FR_636), ENS-PSL, UVSQ, CEA, INSU - CNRS, X, CNES, SU, CNRS, UPCité, LMD, INSU - CNRS, X, IP Paris, ENPC, SU, CNRS, ENS-PSL, PSL, LSCE, UVSQ, INSU - CNRS, CNRS, DRF (CEA), CEA, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS
High-resolution marine data and transient simulations support orbital forcing of ENSO amplitude since the mid-Holocene
Lack of constraint on spatial and long-term temporal variability of the El Niño southern Oscillation (ENSO) and its sensitivity to external forcing limit our ability to evaluate climate models and ENSO future projections. Current knowledge of Holocene ENSO variability derived from paleoclimate reconstructions does not separate the role of insolation forcing from internal climate variability. Using an updated synthesis of coral and bivalve monthly resolved records, we build composite records of seasonality and interannual variability in four regions of the tropical Pacific: Eastern Pacific (EP), Central Pacific (CP), Western Pacific (WP) and South West Pacific (SWP). An analysis of the uncertainties due to the sampling of chaotic multidecadal to centennial variability by short records allows for an objective comparison with transient simulations (mid-Holocene to present) performed using four different Earth System models. Sea surface temperature and pseudo-δ18O are used in model-data comparisons to assess the potential influence of hydroclimate change on records. We confirm the significance of the Holocene ENSO minimum (HEM) 3-6ka compared to low frequency unforced modulation of ENSO, with a reduction of ENSO variance of ∼50 % in EP and ∼80 % in CP. The approach suggests that the increasing trend of ENSO since 6ka can be attributed to insolation, while models underestimate ENSO sensitivity to orbital forcing by a factor of 4.7 compared to data, even when accounting for the large multidecadal variability. Precession-induced change in seasonal temperature range is positively linked to ENSO variance in EP and to a lesser extent in other regions, in both models and observations. Our regional approach yields insights into the past spatial expression of ENSO across the tropical Pacific. In the SWP, today under the influence of the South Pacific Convergence Zone (SPCZ), interannual variability was increased by ∼200 % during the HEM, indicating that SPCZ variability is independent from ENSO on millennial time scales.
(Quaternary Science Reviews. vol. 268, n° 0277-3791, pp. 107125, 01/09/2021)
LOCEAN-VALCO, LOCEAN, MNHN, IRD, INSU - CNRS, SU, CNRS, IPSL (FR_636), ENS-PSL, UVSQ, CEA, INSU - CNRS, X, CNES, SU, CNRS, UPCité, UPCH, LSCE, UVSQ, INSU - CNRS, CNRS, DRF (CEA), CEA, LPG, UA, UN UFR ST, UN, INSU - CNRS, CNRS, MPI-M, AWI, MARUM, UMR ISEM, Cirad, EPHE, PSL, UM, CNRS, EPOC, EPHE, PSL, UB, INSU - CNRS, CNRS, UOR